After the mouse breast cancer 4T1 cells were transduced with GFP-Luc-recombinant retroviruses and HER2-recombinant lentiviruses, the expression of both GFP (luciferase) and HER2 in 4T1-Luc-HER2 cells was detected by flow cytometry and western blot, using 4T1 cells as a negative control. The gene discussed is ERBB2; the disease is breast carcinoma.