We did not observe intermediate values 0 < k < 1 for TP53 variants, while for 3/9 cases in the APC (1), KRAS (1), CDKN2A (1) genes, the normalized VAF was 0.23–0.37, indicating that only subclones of tumor cells carry these somatic variants. This evidence concerns the gene CDKN2A and neoplasm.