To investigate the expression levels of NAF-1, Western blot analysis, real-time PCR, and immunofluorescence were utilized to detect the expression of NAF-1 in several different pancreatic cancer cell lines (Panc-1, MiaPaCa-2, BxPC-3, CF PAC-1, and SW1990) and static pancreatic stellate cell (Q-PSC). This evidence concerns the gene NAF1 and familial pancreatic carcinoma.