Using our nucleated cell model, we used a proteomic dataset from the bone osteosarcoma epithelial U2OS human cell lines with only partial data regarding the PI cycle enzymes and missing the concentration of Gαq, IP3 processing enzymes (IP3Kb and INPP5), OCRL1, PI4K and cPLA2 proteins30 (Supplementary Table S7 online). Here, OCRL is linked to osteosarcoma.