GL261 tumors showed the lowest frequency of KLRG1+ cells and CD25+ cells of the CD4 subset (Supplementary Fig. 11A), whereas 70–80% of CD8 T cells in CT2A were positive for Lag3 and Tim3, markers of dysfunctional T cells in comparison to the other tumor types analyzed (Supplementary Fig. 11A). This evidence concerns the gene CD4 and neoplasm.