As expected, IFN-γ levels were significantly higher in active TB patients (median [IQR]in-house QFN +B = 5.95 IU/ml [1.80–10.00], median [IQR]in-house QFN +C = 4.78 IU/ml [1.53–10.00]) and LTBI individuals (median [IQR]in-house QFN +B = 3.49 IU/ml [1.90–10.00], median [IQR]in-house QFN +C = 4.03 IU/ml [1.25–10.00]) than in healthy controls (median [IQR]in-house QFN +B = 0.02 IU/ml [-0.01–0.07], median [IQR]in-house QFN +C = 0.03 IU/ml [0.00–0.05]) (p < 0.0001) (Fig. 3D). The gene discussed is IFNG; the disease is tuberculosis.