We evaluated the cTnI enrichment performance of the NP and Agarose platforms from sarcomeric extracts containing low amounts of cTnI (<700 ng) obtained from three different human heart samples: a donor heart with normal cardiac function (donor), a heart with dilated cardiomyopathy (diseased), and a postmortem heart with no known history of cardiac dysfunction (postmortem). The gene discussed is TNNI3; the disease is dilated cardiomyopathy.