We further validated this phenomenon in tissue and non-liver cancer cells and found that the amino acid-induced phosphorylation of S6K1 was also significantly repressed in mice expressing a liver-specific Met KO (liver conditional Met-knockout mouse) (Supplementary Fig. S4a) and MET-depleted B16 cells (mouse melanoma) (Supplementary Fig. S4b). This evidence concerns the gene RPS6KB1 and liver cancer.