To explore the function of Nrf2 in mediating ESCC radioresistance in vivo, we established an ESCC xenograft model by subcutaneous injection of wild-type Ec109 cells and Nrf2-overexpressing Ec109 cells, and we treated the tumor-bearing mice with irradiation. The gene discussed is NFE2L2; the disease is esophageal squamous cell carcinoma.