To further our bioinformatics study, we determined the number of methylated CpG in patient-derived cancer line HCT116 (WT) versus HCT116 cells with a double knock-out for DNA-methyltransferases (DNMTs) DNMT1 and DNMT3b. As shown in Fig. 3c, in two independent studies [31, 32], the number of methylated cytosines decreases in MAGE-A promoters when DNA-methyltransferases (DNMT1 and DNMT3b) are depleted. Here, DNMT3B is linked to cancer.