AGT and hypertensive disorder: Guo et al. (2003) demonstrated that angiotensin II-induced hypertension in rats was accompanied by increased tyrosine nitration and thus deactivation of Mn-SOD that serves to augment oxidative stress in the kidney. Activation of NADPH oxidase also induces the production of O2•–, and ONOO– also may induce eNOS uncoupling, leading to overproduction of O2•– as opposed to NO.