After the SW480 and LS1034 cells were transfected with si-KCNQ1OT1#1, si-KCNQ1OT1#2, and si-con, qRT-PCR assay was applied to examine the expression level of KCNQ1OT1 in CRC in vitro. Here, KCNQ1OT1 is linked to colorectal carcinoma.