Compared with non-HCC tissues, Notch1 associates with a higher expression level as well as Notch3, Notch4 60, confirming that the HBx-miR-3188-ZHX2-Notch1 signaling pathway plays an important role in the pathogenesis and progression of HBV-related HCC with a family history of HCC. The gene discussed is NOTCH4; the disease is hepatocellular carcinoma.