Therefore, to determine if DUX4 expression is also linked to the plasma membrane repair deficits in human FSHD myoblasts, we used a 2′-O-methoxyethyl (2′MOE) gapmer antisense oligonucleotide (AON) targeting exon 3 of the DUX4 transcript to reduce DUX4 mRNA. Here, DUX4 is linked to facioscapulohumeral muscular dystrophy.