However, the two-way ANOVA test indicated that ischemia and PDD diets significantly changes the number of proliferating Ki67-positive cells (df = 2, F = 8.816, p = 0.0008), and there were no interaction effects of ischemia and PDD diets in the density of DCX-immunoreactive neuroblasts (Figure 4A,B). The gene discussed is MKI67; the disease is ischemia.