Flow cytometric analysis and immunofluorescent detection of OT-I CD8+ T cells (gated or stained as CD45.1+) in PyMT-OVA tumors demonstrated a marked increase in tumor antigen–specific, exogenously delivered OT-I CD45.1+CD8+ T cells within the tumor parenchyma of PyMT-OVA tumors grown in RaptorECKO mice (Figure 5, G and H). This evidence concerns the gene CD8A and neoplasm.