Our data confirm Fra-1 acts as a transcriptional repressor of CXCL8, IL6, and CTGF in DD myofibroblasts but conversely acts to positively regulate SPFR4, CCL26, and COL6A3 expression, suggesting a highly complex level of regulation of the transcriptional machinery at these genes. Here, COL6A3 is linked to dentin dysplasia.