H19 and hemoglobin measurement: The methylation status of the H19 differentially methylated region (DMR), the loss of heterozygosity (LOH), and the expression of the IGF2 allele in 54 HB tumors was analyzed, and the results showed that 12 cases (22%) had LOH, 9 cases (17%) had loss of imprinting (LOI) [71], and IGF2 was overexpressed in HB.