We found that invasive cells that evolve from low-risk cancer cells overexpress EDB-FN, irrespective of their epithelial or mesenchymal markers; conversely, impeding the invasive abilities of these high-risk cancer cells with a targeted drug abolishes their EDB-FN overexpression, demonstrating a direct correlation between EDB-FN levels and the invasiveness of breast cancer cells. This evidence concerns the gene FN1 and breast cancer.