KLF4 expression in most HCC cells (PLC/PRF/5, Hep3B, Huh7, HepG2, but except SMMC- 7721) was lower than in normal liver cells (L02) not only at mRNA level but also at the protein expression level (Figure 1A and 1B). The gene discussed is KLF4; the disease is hepatocellular carcinoma.