In order to achieve efficient CAR-T cell production, BX-795, an inhibitor of the TBK1/IKKe complex acting downstream of RIG-I, MDA-5, and TLR3, which was previously shown to increase the transduction efficiency of lentivirus to human cell lines including the Jurkat T-cell line and NK92 natural killer cell line [26,27,28], was tested.  α-CD3/α-CD28-stimulated CD3+ T-cells isolated from ALL/NHL patients’ blood were expanded for 10-12 days with CAR-LV of 1 MOI and 6 μM BX-795 for 6 h before washing twice with complete CAR-T media. The gene discussed is TLR3; the disease is acute lymphoblastic leukemia.