GDNF and Wilson disease: The analysis of neurotrophic factors expression in our model showed upregulation of the key neurotrophins such as Ngf, Bdnf and Gdnf. Ngf expression after in vitro WD was biphasic, with significant surge of Ngf expression at D1 and D5 (****P < 0.0001) compared to D0 samples (Figure 3H).