Knockdown of JMJD2D did not affect the expression of p21 and PUMA in human liver cancer Huh-7 cells (Figure S5), which express the p53-Y220C mutant with impaired transactivation activity 16-18, supporting the notion that the effect of JMJD2D on the expression of p53 target genes depends on the intrinsic transcriptional function of p53. This evidence concerns the gene KDM4D and liver cancer.