TP53 and liver cancer: Knockdown of JMJD2D did not affect the expression of p21 and PUMA in human liver cancer Huh-7 cells (Figure S5), which express the p53-Y220C mutant with impaired transactivation activity 16-18, supporting the notion that the effect of JMJD2D on p21 and PUMA expression largely depends on a functional p53.