BBC3 and liver cancer: Knockdown of JMJD2D did not affect the expression of p21 and PUMA in human liver cancer Huh-7 cells (Figure S5), which express the p53-Y220C mutant with impaired transactivation activity 16-18, supporting the notion that the effect of JMJD2D on the expression of p53 target genes depends on the intrinsic transcriptional function of p53.