Further investigating in cultured normal colon epithelial cells, CRC cells and other types of digestive cancer cells revealed that piR-54265 levels was considerably higher in 6 CRC cell lines and their culture medium than in other types of cancer cell lines and the corresponding culture medium (Figure 2E) despite the levels in non-CRC cancer cell lines were slightly higher than that in normal colon epithelial cells. The gene discussed is PIR; the disease is colorectal carcinoma.