Actually, numerous studies have demonstrated that berberine could inhibit HMGB1/Nf-κB pathway in a variety of diseases models, such as brain ischemia, endotoxic shock, and acetaminophen hepatotoxicity (Li et al., 2016; Lee et al., 2017; Zhu et al., 2018). The gene discussed is NFKB1; the disease is brain ischemia.