In the present study, we have assessed the distribution of the seven single nucleotide polymorphisms (SNPs) in the TLR2 (2029C/T, 2258G/A), the TLR4 (896A/G), and the TLR9 (− 1237T/C, − 1486T/C, 1174G/A, and 2848C/T) genes in children and adolescents with acute EBV infection. The gene discussed is TLR4; the disease is Epstein-Barr virus infection.