Previous studies have already showed that (i) glioma-derived extracellular vesicle (EV) can cross the blood brain barrier and be detected in peripheral blood of patients16, (ii) MGMT mRNA is enriched in glioma exosomes (EXOs)17, and (iii) other gene fusion was identified in glioma EXOs18. The gene discussed is MGMT; the disease is central nervous system cancer.