In the context of DNA sensing and viral infection, our data show that although PPP6C deficiency enhanced dsDNA-induced IRF3 activation, PPP6C did not seem to affect p65 phosphorylation or NF-κB-regulated gene expression induced by cytosolic dsDNA, suggesting that PPP6C is not involved in regulating STING-dependent activation of NF-κB. This evidence concerns the gene NFKB1 and viral infectious disease.