To understand whether HUVEC-derived EVs transfer functionally active VE-cadherin to TCs, we isolated EVs from the conditioned medium of GFP-labelled HUVECs co-cultured with mCherry-labelled breast cancer cells, MCF7 or MDA-MB-231 cells, and added them to the naive TCs. The gene discussed is CDH5; the disease is breast carcinoma.