These results were consistent with the expression trend of hub mRNAs in AML compared to normal controls, and were also consistent with the results of multivariable Cox, which the expression of TM6SF, LRRC4, MANSC1, and PYCARD decreased in AML, and the increased expression of ZMAT1 and SLC38A1 in AML samples. Here, PYCARD is linked to acute myeloid leukemia.