Based on our previous results and outcomes from this experiment (decreased number of GFAP+ astrocytes as well as a considerable decrease in the metabolite ratio of mIns/tCr), and according the aforementioned pieces of knowledge (activation of NMDA receptors, increase of Ca2+ influx, cytoskeletal remodelling and the function of astrocytes as a glutamate “trapping” cells), we assume that hHcy enriched by Met diet in combination with IRI 3 days post-ischemia lead to a subacute, partially reversible changes in the number of astrocytes. This evidence concerns the gene GFAP and ischemia.