Using in situ hybridization techniques, compared to control (mice fed with low-fat diet) and obesity resistant mice, obesity prone mice had significantly higher levels of DRD4 mRNA expression in the ventral part of the lateral septal nucleus and ventromedial hypothalamic nucleus, suggesting a role for this receptor in the hypothalamic pathway [242]. The gene discussed is DRD4; the disease is obesity due to melanocortin 4 receptor deficiency.