Our present data evidenced that diabetes decreased BAG3, Bcl-2, Nrf-2 and HO-1 expression, further reduced myocardial IR-depressed BAG3, Bcl-2, Nrf-2 and HO-1 expression and increased cleavage caspase 3/PARP, leading to apoptosis formation in the heart. This evidence concerns the gene BAG3 and diabetes mellitus.