PRDX1 and ischemia: Using immunoblotting, the alteration in the amount of the recombinant Prx1 and Prx2 in the blood serum of animals was analyzed in the early period following protein administration (a 15 min interval was chosen, which corresponds to the beginning of ischemia, according to a previously described animal model), as well as after a long time (6 h) following intravenous introduction (Figure 2 and Figure 3).