The sensitivity of our PNA-mediated PCR clamping method was assessed using a range with different percentages, from 50 to 0.1%, of the p.Thr790Met alteration of EGFR. Three ranges were realized by diluting an EGFR p.Thr790Met reference standard, 50% mutated, in wild-type DNA extracted from lymphocytes of healthy donors to avoid cancer cell aneuploidy, which would perturb the precision of our dilutions. This evidence concerns the gene EGFR and cancer.