Fig 2A demonstrates that effective intracellular neutralisation is mediated by anti-DLP antibodies. To test whether this neutralization is physiologically relevant and capable of providing protective immunity in vivo we performed infection and re-challenge experiments. Importantly, the data show that mice immunized with DLPs were protected from subsequent challenge with Epizootic Disease of Infant Mice (EDIM) virus (Fig 2B), with statistically significant differences in viral loads detected on days 3–6 post infection. This confirmed the importance of anti-VP6 antibodies in vivo. Here, TXNL4B is linked to infection.