We knocked down KIF2C in its high-expressing HCC cell lines SK-Hep1 and SNU449 using small hairpin RNA (shRNA), and overexpressed KIF2C in its low-expressing HepG2 and SNU387 cells, and the efficiencies of KIF2C silencing and overexpression were confirmed by Western blot (Fig. S2A and S2B). This evidence concerns the gene KIF2C and hepatocellular carcinoma.