HOXB6 and Alzheimer disease: To further explore AD-associated hypermethylation in this gene, we used pyrosequencing to validate our HOXB6 DMR, covering 2 CpG sites on the array (cg17179862, cg03803541) as well as 3 neighboring CpG sites that were not covered by the 450K array (chr17:46681421, chr17:46681394, and chr17:46681383).