To further confirm the role of DC and other myeloid cells in controlling LVS during early stages of infection, splenocytes from naïve and LVS-infected WT mice were depleted of B and T cells and sorted by flow cytometry to purify total DC (live CD45+ CD11c+ MHCII+ cells) and neutrophils (live CD11c- MHCII- CD11b+ Ly6G+ cells; gating strategy shown in S2 Fig). The gene discussed is ITGAM; the disease is infection.