When the global gene expression changes between hiPSC-derived trophoblasts from CZS-affected and non-affected twins were analyzed before ZIKVBR infection, only 4 differentially expressed genes were found, being two of them upregulated (CDRT4/CMT1A duplicated region transcript 4 and TMEM176A/Transmembrane protein 176A) and two downregulated (TGFB2-OT1/TGFB2 overlapping transcript 1 and HIST2H4B/histone cluster 2 H4 family member b) in the CZS-affected twins. This evidence concerns the gene TMEM176A and infection.