TWIST1 and glioblastoma: To verify the hypothesis that EP may affect the migration and invasion ability of glioblastoma cells by regulating the EMT process, western blot was used to detect the protein expression levels of EMT-related molecules, including epithelial marker E-cadherin, mesenchymal marker Vimentin and EMT-related transcription factors Snail, Twist1 and ZEB1 in U251 and U87 cells after EP treatment at different concentrations for 48 h.