Finally, to verify whether EP regulates the process of EMT through NF-κB and ERK pathway and thus inhibits the migration and invasion of glioblastoma cells, the protein expression of E-cadherin, Vimentin, Snail, Twist1 and ZEB1 in control group, TNF-α treatment group, TNF-α Coupled with U0126 Treatment Group, TNF-α coupled with EP treatment group was detected. This evidence concerns the gene VIM and glioblastoma.