Logistic regression analysis revealed that upregulated SPC25 expression in HCC was closely associated with age (odds risk [OR] = 0.568 for ≥65 vs <65, 95% CI [0.372–0.863], P = 0.0083), pathological stage (OR = 1.957 for stage III + stage IV vs stage I + stage II, 95% CI [1.202–3.223], P = 0.0075), histologic grade (OR= 4.936 for grade 3 vs grade 1, 95% CI [2.506–10.104], P < 0.001; OR= 12.187 for grade 4 vs grade 1, 95% CI [2.827–85.344], P = 0.0026), and patients survival status (OR = 1.958 for dead vs. alive, 95% CI [1.272–3.036], P = 0.0024) (Table 2). The gene discussed is SPC25; the disease is hepatocellular carcinoma.