Twenty-six (35.1%) of 74 tumors in hepatocellular carcinoma had elevated levels of PD-L1 protein as detected by IHC, whereas high PD-L1 expression significantly associated with low alanine transaminase (ALT) levels (p=0.037) and high Edmondson grading (P<0.001) (Table 2). This evidence concerns the gene CD274 and hepatocellular carcinoma.