Previous researches demonstrated that CAR-T cell composed of CD8α, intracellular signaling domains, 4-1BB, and CD3ζ, the anti-GPC3 scFv, hinge and CD28 transmembrane could specifically lyse HCC cell lines, and significantly suppressing growth in immunodeficient mice, of orthotopic xenografts 60. The gene discussed is CD28; the disease is hepatocellular carcinoma.