KLK3 and Familial prostate cancer: According to the guideline of National Comprehensive Cancer Network (NCCN) for prostate cancer, the high-risk (HR) PCa is defined as T3a, PSA>20ng/ml, or Gleason Score (GS 8-10), and Very high-risk (VHR) as T3b-4, grade group 5, or >4 core positive grade group 4-5 9.