Through luciferase reporter and RIP assays we were able to confirm that lncZEB1-AS1 can directly interact with miR-302b in HCC cells, and we were additionally able to confirm that lncZEB1-AS1 knockdown significantly increased miR-302b expression in these HCC cells, thereby resulting in EGFR downregulation. This evidence concerns the gene EGFR and hepatocellular carcinoma.