In this study, we found that social isolation of C57BL/6J mice (a) increased anxiety-like behavior; (b) impaired synaptic (phasic) and extrasynaptic (tonic) GABAergic inhibitory neurotransmission; (c) reduced hippocampal ATP levels and gephyrin expression, and (d) these behavioral deficits, and cellular and molecular impairments were attenuated with DHM administration. Here, GPHN is linked to Anxiety.