SRM showed robust concordance of increased expression of all selected protein in TIF samples, and increased abundance of CD14, TBG, and TSP1 (THBS1) in ccRCC serum samples relative to a healthy control serum samples, while ELISA showed elevated abundance of ENO2 and TSP1 in ccRCC patient-derived serum. The gene discussed is ENO2; the disease is nonpapillary renal cell carcinoma.