We investigated whether the changes of USP18 expression could affect the chemotherapy sensitivity of colorectal cancer cells using three common chemotherapeutic molecular drugs, including fluorouracial, doxorubicin and cisplatin Different concentrations of fluorouracial (0, 20, 40, 60, and 80 g/mL), doxorubicin (0, 0.5, 2.5, 5, and 10 μM), and cisplatin (0, 10, 20, 30, and 40 μM) were applied in this study. Here, USP18 is linked to colorectal cancer.