To investigate whether STK4 caused β‐catenin phosphorylation, and the phosphorylated β‐catenin was then degraded through proteasomal degradation pathway, colon cancer cells expressing GFP‐STK4 or GFP‐empty plasmids were treated with a proteasome inhibitor (MG‐132) for 6 and 12 h following by western blotting to evaluate the expression of phosphorylated β‐catenin. This evidence concerns the gene STK4 and colonic neoplasm.